Which of the following is a safe lifting practice required by DA PAM 385-64?

Ensuring that items are secured together when lifting is a critical safe lifting practice outlined in DA PAM 385-64. This practice minimizes the risk of dropped loads or shifting items that could lead to injury. When items are secured properly, it provides better control during lifting and moving, reducing the likelihood of accidents.

The other choices may also contribute to safety in handling but do not specifically align with the principle emphasized in the context of DA PAM 385-64 regarding safe lifting practices. Lifting with a partner can enhance safety in certain situations, but it is not always practical or necessary for every lift. Wearing gloves protects against cuts and abrasions; however, it does not directly address the safe lifting technique. Using mechanical aids is a great method for reducing physical strain on the body, but it is not a mandatory requirement for all lifting tasks. Therefore, while those practices are important, ensuring items are secured together when lifting is the practice that directly correlates with the guidelines established by DA PAM 385-64.